Composite

Part:BBa_K3002216:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + L1_connector_Pos2 + ccA_MHETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2660
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
    Illegal NotI site found at 4073
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4983
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3671
    Illegal NgoMIV site found at 4132
    Illegal NgoMIV site found at 4162
    Illegal NgoMIV site found at 4477
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (spectinomycin resistance) and BBa_K3002113 (cCA_wildtype MHETase_HA).

References